SOX17 Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: TA500281S

Anti-Sox17 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. (TA500281). Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. (TA500281). Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.

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  Anti-SOX17 mouse monoclonal antibody (TA500281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17 (RC220888).

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Specifications

Product Data

• Clone Name: OTI2B6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000; IHC 1:50
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 177-414 of human SOX17
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.15 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: SRY-box transcription factor 17
• Database Link:
  NP_071899
  Entrez Gene 20671 MouseEntrez Gene 312936 RatEntrez Gene 486955 DogEntrez Gene 64321 Human
  UniProt ID Q9H6I2
• Background: SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins
• Synonyms: VUR3

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Wnt signaling pathway