Prominin 2 (PROM2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5E5]

CAT#: TA500310AM

Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI5E5 (formerly 5E5), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Positive lysates LY403405 (100ug) and LC403405 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PROM2 monoclonal antibody (1:200).

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  Anti-PROM2 mouse monoclonal antibody (TA500310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROM2 (RC211605).

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  Immunoprecipitation (IP) of PROM2 by using TrueMab monoclonal anti-PROM2 antibodies (Negative control: IP without adding anti-PROM2 antibody.). For each experiment, 500ul of DDK tagged PROM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PROM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Specifications

Product Data

• Clone Name: OTI5E5
• Applications: IF, IP, WB
• Recommended Dilution: WB 1:2000, IP: 4ug/mL
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human PROM2 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91.8 kDa
• Gene Name: prominin 2
• Database Link:
  NP_653308
  Entrez Gene 150696 Human
  UniProt ID Q8N271
• Synonyms: PROML2

Reference Data

• Protein Families: Transmembrane