USP13 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4G11]

CAT#: TA500318AM

Anti-USP13 mouse monoclonal antibody, clone OTI4G11 (formerly 4G11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY USP13 (Cat# RC202190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13(Cat# TA500318). Positive lysates LY401292 (100ug) and LC401292 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-USP13 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-USP13mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunoprecipitation (IP) of USP13 by using TrueMab monoclonal anti-USP13 antibodies (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-USP13 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI4G11
• Applications: IHC, IP, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IP: 4ug/mL
• Reactivities: Human, Monkey, Dog, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human USP13 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97.3 kDa
• Gene Name: ubiquitin specific peptidase 13
• Database Link:
  NP_003931
  Entrez Gene 72607 MouseEntrez Gene 310306 RatEntrez Gene 478640 DogEntrez Gene 708624 MonkeyEntrez Gene 8975 Human
  UniProt ID Q92995
• Synonyms: IsoT-3; ISOT3

Reference Data

• Protein Families: Druggable Genome, Protease