GAD67 (GAD1) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

CAT#: TA500328

GAD1 (GAD67) mouse monoclonal antibody, clone OTI3H2 (formerly 3H2)

Product Images

Anti-GAD1 mouse monoclonal antibody (TA500328) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAD1 (RC207226).

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500328)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500328)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500328)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500328)

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500328)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1. Positive lysates LY400290 (100ug) and LC400290 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1 monoclonal antibody.

• Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI3H2
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:500-1:1000, IHC 1:50, IF 1:100, IP: 4ug/mL
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.46 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.9 kDa
• Gene Name: glutamate decarboxylase 1
• Database Link:
  NP_000808
  Entrez Gene 14415 MouseEntrez Gene 24379 RatEntrez Gene 478794 DogEntrez Gene 2571 Human
  UniProt ID Q99259
• Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
• Synonyms: CPSQ1; GAD; SCP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus