SIGLEC9 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8F7]

CAT#: TA500380BM

SIGLEC9 mouse monoclonal antibody, clone OTI8F7 (formerly 8F7), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9. Positive lysates LY402335 (100ug) and LC402335 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIGLEC9 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500380)

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Specifications

Product Data

• Clone Name: OTI8F7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2500~5000, IHC 1:50
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIGLEC9 (NP_055256) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: sialic acid binding Ig like lectin 9
• Database Link:
  NP_055256
  Entrez Gene 719831 MonkeyEntrez Gene 27180 Human
  UniProt ID Q9Y336
• Background: Putative adhesion molecule that mediates sialic-acid dependent binding to cells.Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
• Synonyms: CD329; CDw329; FOAP-9; OBBP-LIKE; siglec-9

Reference Data

• Protein Families: Druggable Genome, Transmembrane