SIGLEC9 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6D8]

CAT#: TA500382AM

SIGLEC9 mouse monoclonal antibody, clone OTI6D8 (formerly 6D8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9. Positive lysates LY402335 (100ug) and LC402335 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIGLEC9 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500382)

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  Immunofluorescent staining of HT29 cells using anti-SIGLEC9 mouse monoclonal antibody (TA500382).

Specifications

Product Data

• Clone Name: OTI6D8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:50, IF 1:50
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIGLEC9 (NP_055256) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: sialic acid binding Ig like lectin 9
• Database Link:
  NP_055256
  Entrez Gene 719831 MonkeyEntrez Gene 27180 Human
  UniProt ID Q9Y336
• Background: Putative adhesion molecule that mediates sialic-acid dependent binding to cells.Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
• Synonyms: CD329; CDw329; FOAP-9; OBBP-LIKE; siglec-9

Reference Data

• Protein Families: Druggable Genome, Transmembrane