CD4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6E10]

CAT#: TA500481BM

Anti-CD4 mouse monoclonal antibody, clone OTI6E10 (formerly 6E10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Positive lysates LY400209 (100ug) and LC400209 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CD4 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD4 mouse monoclonal antibody. (TA500481)

Specifications

Product Data

• Clone Name: OTI6E10
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human CD4 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.1 kDa
• Gene Name: CD4 molecule
• Database Link:
  NP_000607
  Entrez Gene 920 Human
  UniProt ID P01730
• Background: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts
• Synonyms: CD4mut

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway