MRI1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6A10]

CAT#: TA500490BM

MRI1 mouse monoclonal antibody, clone OTI6A10 (formerly 6A10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRI1 (RC200993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRI1. Positive lysates LY422176 (100ug) and LC422176 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MRI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500490)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500490)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500490)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500490)

• Anti-MRI1 mouse monoclonal antibody (TA500490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRI1 (RC200993).

• Immunofluorescent staining of A549 cells using anti-MRI1 mouse monoclonal antibody (TA500490).

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Specifications

Product Data

• Clone Name: OTI6A10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRI1 (NP_115661) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.1 kDa
• Gene Name: methylthioribose-1-phosphate isomerase 1
• Database Link:
  NP_001026897
  Entrez Gene 84245 Human
  UniProt ID Q9BV20
• Background: Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) (By similarity.
• Synonyms: MRDI; MTNA; Ypr118w