SCY1 like 3 (SCYL3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4F10]

CAT#: TA500493BM

Anti-SCYL3 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3. Positive lysates LY412474 (100ug) and LC412474 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCYL3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500493, Dilution 1:50)

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  Anti-SCYL3 mouse monoclonal antibody (TA500493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCYL3 (RC204859).

Specifications

Product Data

• Clone Name: OTI4F10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000 IHC 1:50 IF 1:100 Flow 1:1,000
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SCYL3 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.8 kDa
• Gene Name: SCY1 like pseudokinase 3
• Database Link:
  NP_065156
  Entrez Gene 701768 MonkeyEntrez Gene 57147 Human
  UniProt ID Q8IZE3
• Background: May play a role in regulating cell adhesion/migration complexes in migrating cells
• Synonyms: PACE-1; PACE1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase