Kindlin 2 (FERMT2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9E4]

CAT#: TA500504AM

Anti-FERMT2 mouse monoclonal antibody, clone OTI9E4 (formerly 9E4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FERMT2 (Cat# RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2(Cat# TA500504). Positive lysates LY416377 (100ug) and LC416377 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FERMT2-Knockout HeLa cells (KO, Cat# LC812552) were separated by SDS-PAGE and immunoblotted with anti-FERMT2 monoclonal antibody TA500504 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500504).

• Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI9E4
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:50, IP 2ug/500ul
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.9 kDa
• Gene Name: FERM domain containing kindlin 2
• Database Link:
  NP_006823
  Entrez Gene 218952 MouseEntrez Gene 289992 RatEntrez Gene 490693 DogEntrez Gene 693958 MonkeyEntrez Gene 10979 Human
  UniProt ID Q96AC1
• Background: Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
• Synonyms: KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B