VMAT2 (SLC18A2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9E11]

CAT#: TA500506AM

Anti-SLC18A2 (VMAT2) mouse monoclonal antibody, clone OTI9E11 (formerly 9E11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC18A2 (RC221342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC18A2. Positive lysates LY401066 (100ug) and LC401066 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SLC18A2 monoclonal antibody (1:200).

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  Figure from citation: Western Blot of SLC18A2 protein level by using anti-SLC18A2 (9E11) antibody in the striatum of autopsied human brain. View Citation

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC18A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500506)

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  Anti-SLC18A2 mouse monoclonal antibody (TA500506) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC18A2 (RC221342).

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  HEK293T cells transfected with either RC221342 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC18A2 antibody (TA500506), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9E11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector .
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.7 kDa
• Gene Name: solute carrier family 18 member A2
• Database Link:
  NP_003045
  Entrez Gene 25549 RatEntrez Gene 214084 MouseEntrez Gene 6571 Human
  UniProt ID Q05940
• Background: The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs,including reserpine and tetrabenazine.
• Synonyms: SVAT; SVMT; VAT2; VMAT2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Parkinson's disease