TMEM100 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F8]

CAT#: TA500508BM

Anti-TMEM100 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (RC203980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100. Positive lysates LY402665 (100ug) and LC402665 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TMEM100 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-TMEM100 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-TMEM100mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI1F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000 IHC 1:50
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human TMEM100 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: transmembrane protein 100
• Database Link:
  NP_060756
  Entrez Gene 609661 DogEntrez Gene 707032 MonkeyEntrez Gene 55273 Human
  UniProt ID Q9NV29
• Synonyms: FLJ10970; FLJ37856

Reference Data

• Protein Families: Transmembrane