NY-ESO-1 (CTAG1B) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C9]

CAT#: TA500519AM

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B. Positive lysates LY400527 (100ug) and LC400527 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500519)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500519)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500519)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500519)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500519)

• Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CTAG1B (RC213318) using anti-CTAG1B antibody (TA500519/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

• Immunofluorescent staining of A549 cells using anti-CTAG1B mouse monoclonal antibody (TA500519).

• Flow cytometric analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (PS100001, Blue) using anti-CTAG1B antibody (TA500519). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• Flow cytometric analysis of living NCI-H1299 cells, using anti-CTAG1B antibody (TA500519, Red), compared to an isotype control (green) (1:100).

• Flow cytometric analysis of living A375 cells, using anti-CTAG1B antibody (TA500519, Red), compared to an isotype control (green) (1:100).

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Specifications

Product Data

• Clone Name: OTI3C9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTAG1B (NP_001318) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.8 kDa
• Gene Name: cancer/testis antigen 1B
• Database Link:
  NP_001318
  Entrez Gene 1485 Human
  UniProt ID P78358
• Background: Cancer/testis antigens, such as CTAG1B, are expressed in a variety of malignant tumors but soley in testis among normal adult tissues (Yoshida et al., 2006 [PubMed 16596224]). [supplied by OMIM]
• Synonyms: CT6.1; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1

Reference Data

• Protein Families: Druggable Genome