FRK Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6D2]

CAT#: TA500526AM

FRK mouse monoclonal antibody, clone OTI6D2 (formerly 6D2), Biotinylated

Product Images

HEK293T cells transfected with either RC204460 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FRK antibody (TA500526), and then analyzed by flow cytometry.

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  Anti-FRK mouse monoclonal antibody (TA500526) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK (RC204460).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500526)

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Specifications

Product Data

• Clone Name: OTI6D2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.1 kDa
• Gene Name: fyn related Src family tyrosine kinase
• Database Link:
  NP_002022
  Entrez Gene 14302 MouseEntrez Gene 79209 RatEntrez Gene 2444 Human
  UniProt ID P42685
• Background: The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq]
• Synonyms: GTK; PTK5; RAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase