GBP2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1E5]

CAT#: TA500657BM

GBP2 mouse monoclonal antibody, clone OTI1E5 (formerly 1E5), HRP conjugated

Product Images

HEK293T cells transfected with either RC209939 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody (TA500657), and then analyzed by flow cytometry.

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  Anti-GBP2 mouse monoclonal antibody (TA500657) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2 (RC209939).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500657)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (RC209939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2. Positive lysates LY401329 (100ug) and LC401329 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; HT29: human; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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Specifications

Product Data

• Clone Name: OTI1E5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67 kDa
• Gene Name: guanylate binding protein 2
• Database Link:
  NP_004111
  Entrez Gene 2634 Human
  UniProt ID P32456
• Background: Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP. [provided by RefSeq]
• Synonyms: DKFZp451C2311