GBP2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D1]

CAT#: TA500670AM

GBP2 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (RC209939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2. Positive lysates LY401329 (100ug) and LC401329 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-GBP2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500670)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500670)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500670)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500670)

• Anti-GBP2 mouse monoclonal antibody (TA500670) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2 (RC209939).

• HEK293T cells transfected with either RC209939 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody (TA500670), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:100~200, WB: 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67 kDa
• Gene Name: guanylate binding protein 2
• Database Link:
  NP_004111
  Entrez Gene 2634 Human
  UniProt ID P32456
• Background: Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP. [provided by RefSeq]
• Synonyms: DKFZp451C2311