FH Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F10]

CAT#: TA500675BM

FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH. Positive lysates LY400053 (100ug) and LC400053 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500675)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500675)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500675)

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  Anti-FH mouse monoclonal antibody (TA500675) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH (RC200614).

• HEK293T cells transfected with either RC200614 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody (TA500675), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1F10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.2 kDa
• Gene Name: fumarate hydratase
• Database Link:
  NP_000134
  Entrez Gene 24368 RatEntrez Gene 14193 MouseEntrez Gene 480092 DogEntrez Gene 2271 Human
  UniProt ID P07954
• Background: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]
• Synonyms: FMRD; HLRCC; LRCC; MCL; MCUL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma