PSMA7 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI8F9]

CAT#: TA500694AM

Anti-PSMA7 mouse monoclonal antibody, clone OTI8F9 (formerly 8F9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA7 (RC201169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA7. Positive lysates LY400987 (100ug) and LC400987 (20ug) can be purchased separately from OriGene.

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  Anti-PSMA7 mouse monoclonal antibody (TA500694) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMA7 (RC201169).

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  Flow cytometric analysis of Jurkat cells, using anti-PSMA7 antibody (TA500694), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

Specifications

Product Data

• Clone Name: OTI8F9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMA7 (NP_002783) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.9 kDa
• Gene Name: proteasome 20S subunit alpha 7
• Database Link:
  NP_002783
  Entrez Gene 26444 MouseEntrez Gene 29674 RatEntrez Gene 5688 Human
  UniProt ID O14818
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.
• Synonyms: C6; HEL-S-276; HSPC; RC6-1; XAPC7

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome