IBRDC2 (RNF144B) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C2]

CAT#: TA500696AM

RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI3C2 (formerly 3C2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B. Positive lysates LY405351 (100ug) and LC405351 (20ug) can be purchased separately from OriGene.

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  Anti-RNF144B mouse monoclonal antibody (TA500696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B (RC209302).

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  Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500696).

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  HEK293T cells transfected with either RC209302 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNF144B antibody (TA500696), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody (TA500696), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody (TA500696), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3C2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: ring finger protein 144B
• Database Link:
  NP_877434
  Entrez Gene 218215 MouseEntrez Gene 255488 Human
  UniProt ID Q7Z419
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2

Reference Data

• Protein Families: Transmembrane