Sorbitol Dehydrogenase (SORD) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1D5]

CAT#: TA500702BM

Anti-SORD (Sorbitol Dehydrogenase) mouse monoclonal antibody, clone OTI1D5 (formerly 1D5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100ug) and LC401082 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SORD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Anti-SORD mouse monoclonal antibody (TA500702) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SORD (RC200415).

• 

  Immunofluorescent staining of HepG2 cells using anti-SORD mouse monoclonal antibody (TA500702).

• 

  HEK293T cells transfected with either pCMV6-ENTRY SORD (RC200415) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SORD mouse monoclonal (TA500702, Dilution 1:1,000), and then analyzed by flow cytometry.

• 

  Flow cytometric analysis of Hela cells, using anti-SORD antibody (TA500702), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

• Flow cytometric analysis of Jurkat cells, using anti-SORD antibody (TA500702), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI1D5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.3 kDa
• Gene Name: sorbitol dehydrogenase
• Database Link:
  NP_003095
  Entrez Gene 6652 Human
  UniProt ID Q00796
• Synonyms: HEL-S-95n; SORD1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways