TTLL12 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3H6]

CAT#: TA500776AM

TTLL12 mouse monoclonal antibody, clone OTI3H6 (formerly 3H6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates LY402417 (100ug) and LC402417 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TTLL12 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500776)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500776)

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  Anti-TTLL12 mouse monoclonal antibody (TA500776) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12 (RC200841).

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  HEK293T cells transfected with either RC200841 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTLL12 antibody (TA500776), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-TTLL12 antibody (TA500776), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3H6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.2 kDa
• Gene Name: tubulin tyrosine ligase like 12
• Database Link:
  NP_055955
  Entrez Gene 300105 RatEntrez Gene 474474 DogEntrez Gene 714871 MonkeyEntrez Gene 23170 Human
  UniProt ID Q14166
• Synonyms: dJ526I14.2