TTLL12 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5H3]

CAT#: TA500780BM

Anti-TTLL12 mouse monoclonal antibody, clone OTI5H3 (formerly 5H3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates LY402417 (100ug) and LC402417 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TTLL12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TTLL12-Knockout 293T cells (KO, Cat# LC811864) were separated by SDS-PAGE and immunoblotted with anti-TTLL12 monoclonal antibody TA500780, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500780, Dilution 1:50)

• Anti-TTLL12 mouse monoclonal antibody (TA500780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12 (RC200841).

• HEK293T cells transfected with either RC200841 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTLL12 antibody (TA500780), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow: 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.4 kDa
• Gene Name: tubulin tyrosine ligase like 12
• Database Link:
  NP_055955
  Entrez Gene 300105 RatEntrez Gene 474474 DogEntrez Gene 23170 Human
  UniProt ID Q14166
• Synonyms: dJ526I14.2