ERAB (HSD17B10) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5E2]

CAT#: TA500813BM

Anti-HSD17B10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (RC201734, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10. Positive lysates LY401426 (100ug) and LC401426 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSD17B10 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-HSD17B10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500813, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSD17B10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500813, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSD17B10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500813, Dilution 1:50)

• 

  Anti-HSD17B10 mouse monoclonal antibody (TA500813) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B10 (RC201734).

• Flow cytometric analysis of Hela cells, using anti-HSD17B10 antibody (TA500813), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI5E2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSD17B10 (NP_004484) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.9 kDa
• Gene Name: hydroxysteroid 17-beta dehydrogenase 10
• Database Link:
  NP_004484
  Entrez Gene 15108 MouseEntrez Gene 63864 RatEntrez Gene 700018 MonkeyEntrez Gene 3028 Human
  UniProt ID Q99714
• Background: This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
• Synonyms: 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Metabolic pathways, Valine, leucine and isoleucine degradation