MAN1 (LEMD3) Mouse Monoclonal Antibody [Clone ID: OTI3C8]

CAT#: TA500815S

LEMD3 (MAN1) mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3. Positive lysates LY402311 (100ug) and LC402311 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.

• 

  Flow cytometric Analysis of Hela cells, using anti-LEMD3 antibody (TA500815), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-LEMD3 antibody (TA500815), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI3C8
• Applications: WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.83 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 99.8 kDa
• Gene Name: LEM domain containing 3
• Database Link:
  NP_055134
  Entrez Gene 380664 MouseEntrez Gene 680066 RatEntrez Gene 717597 MonkeyEntrez Gene 23592 Human
  UniProt ID Q9Y2U8
• Background: This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.
• Synonyms: MAN1

Reference Data

• Protein Families: Transmembrane