PPME1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7F12]

CAT#: TA500823BM

Anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1. Positive lysates LY402509 (100ug) and LC402509 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500823, Dilution 1:50)

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  Anti-PPME1 mouse monoclonal antibody (TA500823) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1 (RC200009).

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  Flow cytometric analysis of Hela cells, using anti-PPME1 antibody (TA500823), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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  Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody (TA500823), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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Specifications

Product Data

• Clone Name: OTI7F12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.3 kDa
• Gene Name: protein phosphatase methylesterase 1
• Database Link:
  NP_057231
  Entrez Gene 72590 MouseEntrez Gene 361613 RatEntrez Gene 51400 Human
  UniProt ID Q9Y570
• Background: Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit
• Synonyms: PME-1

Reference Data

• Protein Families: Druggable Genome