Aconitase 2 (ACO2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7A11]

CAT#: TA500841AM

Anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7A11 (formerly 7A11), Biotinylated

Product Images

Anti-ACO2 mouse monoclonal antibody (TA500841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2 (RC204307).

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  HEK293T cells transfected with either pCMV6-ENTRY ACO2 (RC204307) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ACO2 mouse monoclonal (TA500841), and then analyzed by flow cytometry.

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  Flow cytometric analysis of Jurkat cells, using anti-ACO2 antibody (TA500841), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2. Positive lysates LY400442 (100ug) and LC400442 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of Hela cells, using anti-ACO2 antibody (TA500841), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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Specifications

Product Data

• Clone Name: OTI7A11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.4 kDa
• Gene Name: aconitase 2
• Database Link:
  NP_001089
  Entrez Gene 11429 MouseEntrez Gene 79250 RatEntrez Gene 50 Human
  UniProt ID Q99798
• Background: The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
• Synonyms: ACONM; HEL-S-284; ICRD; OCA8; OPA9

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways