GRASP1 (GRIPAP1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1E3]

CAT#: TA500876BM

Anti-GRIPAP1 (GRASP1) mouse monoclonal antibody, clone OTI1E3 (formerly 1E3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1. Positive lysates LY402754 (100ug) and LC402754 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500876, Dilution 1:50)

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  Flow cytometric analysis of Jurkat cells, using anti-GRIPAP1 antibody (TA500876), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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  Flow cytometric analysis of Hela cells, using anti-GRIPAP1 antibody (TA500876), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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  Immunofluorescent staining of HepG2 cells using anti-GRIPAP1 mouse monoclonal antibody (TA500876).

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  Anti-GRIPAP1 mouse monoclonal antibody (TA500876) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GRIPAP1 (RC210936).

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Specifications

Product Data

• Clone Name: OTI1E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRIPAP1 (NP_064522) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.0 kDa
• Gene Name: GRIP1 associated protein 1
• Database Link:
  NP_064522
  Entrez Gene 54645 MouseEntrez Gene 116493 RatEntrez Gene 56850 Human
  UniProt ID Q4V328
• Background: This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). In brain studies, the encoded protein was found with the GRIP/AMPA receptor complex. Multiple alternatively spliced transcript variants have been described that encode different protein isoforms; however, the full-length nature and biological validity of all of these variants have not been determined
• Synonyms: GRASP-1

Reference Data

• Protein Families: Druggable Genome