RALBP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI11B2]

CAT#: TA500893AM

Anti-RALBP1 (RIP1) mouse monoclonal antibody, clone OTI11B2 (formerly 11B2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1. Positive lysates LY402031 (100ug) and LC402031 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RALBP1-Knockout HeLa cells (KO, Cat# LC833874) were separated by SDS-PAGE and immunoblotted with anti-RALBP1 monoclonal antibody TA500893 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Anti-RALBP1 mouse monoclonal antibody (TA500893) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1 (RC201524).

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  Immunoprecipitation (IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either pCMV6-ENTRY RALBP1 (RC201524) (Red) or empty vector control plasmid (Blue) were immunostained with anti-RALBP1 mouse monoclonal (TA500893), and then analyzed by flow cytometry.

• Flow cytometric analysis of Jurkat cells, using anti-RALBP1 antibody (TA500893), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

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Specifications

Product Data

• Clone Name: OTI11B2
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100, IP: 4ug/mL
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.0 kDa
• Gene Name: ralA binding protein 1
• Database Link:
  NP_006779
  Entrez Gene 19765 MouseEntrez Gene 84014 RatEntrez Gene 490548 DogEntrez Gene 10928 Human
  UniProt ID Q15311
• Background: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin
• Synonyms: RIP1; RLIP1; RLIP76

Reference Data

• Protein Pathways: Pancreatic cancer, Pathways in cancer