Pleckstrin (PLEK) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2D11]

CAT#: TA500900AM

Anti-PLEK (Pleckstrin) mouse monoclonal antibody, clone OTI2D11 (formerly 2D11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK. Positive lysates LY419171 (100ug) and LC419171 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either pCMV6-ENTRY PLEK (RC203780) (Red) or empty vector control plasmid (Blue) were immunostained with anti-PLEK mouse monoclonal (TA500900), and then analyzed by flow cytometry.

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  Flow cytometric analysis of Jurkat cells, using anti-PLEK antibody (TA500900), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

Specifications

Product Data

• Clone Name: OTI2D11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PLEK (NP_002655) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.1 kDa
• Gene Name: pleckstrin
• Database Link:
  NP_002655
  Entrez Gene 56193 MouseEntrez Gene 364206 RatEntrez Gene 5341 Human
  UniProt ID P08567
• Background: Major protein kinase C substrate of platelets, its exact function is not known
• Synonyms: P47

Reference Data

• Protein Families: Druggable Genome