CISD1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3A1]

CAT#: TA500905BM

Anti-CISD1 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1. Positive lysates LY413042 (100ug) and LC413042 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500905, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CISD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500905, Dilution 1:50)

• 

  Anti-CISD1 mouse monoclonal antibody (TA500905) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1 (RC203308).

Specifications

Product Data

• Clone Name: OTI3A1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.2 kDa
• Gene Name: CDGSH iron sulfur domain 1
• Database Link:
  NP_060934
  Entrez Gene 52637 MouseEntrez Gene 294362 RatEntrez Gene 55847 Human
  UniProt ID Q9NZ45
• Background: CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity
• Synonyms: C10orf70; MDS029; mitoNEET; ZCD1

Reference Data

• Protein Families: Transmembrane