RALBP1 Mouse Monoclonal Antibody [Clone ID: OTI11C6]

CAT#: TA500908

Anti-RALBP1 (RIP1) mouse monoclonal antibody, clone OTI11C6 (formerly 11C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1. Positive lysates LY402031 (100ug) and LC402031 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-RALBP1 mouse monoclonal antibody (TA500908) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1 (RC201524).

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  Immunoprecipitation (IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC201524 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RALBP1 antibody (TA500908), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI11C6
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100, IP: 4ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.25 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.9 kDa
• Gene Name: ralA binding protein 1
• Database Link:
  NP_006779
  Entrez Gene 19765 MouseEntrez Gene 84014 RatEntrez Gene 10928 Human
  UniProt ID Q15311
• Synonyms: RIP1; RLIP1; RLIP76

Reference Data

• Protein Pathways: Pancreatic cancer, Pathways in cancer