IDH3A Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2E9]

CAT#: TA500912AM

Anti-IDH3A mouse monoclonal antibody, clone OTI2E9 (formerly 2E9), Biotinylated

Product Images

Anti-IDH3A mouse monoclonal antibody (TA500912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A (RC200313).

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  HEK293T cells transfected with either pCMV6-ENTRY IDH3A (RC200313) (Red) or empty vector control plasmid (Blue) were immunostained with anti-IDH3A mouse monoclonal (TA500912), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E9
• Applications: FC, IF
• Recommended Dilution: IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.6 kDa
• Gene Name: isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha
• Database Link:
  NP_005521
  Entrez Gene 67834 MouseEntrez Gene 114096 RatEntrez Gene 3419 Human
  UniProt ID P50213
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Synonyms: H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase [NAD] subunit alpha; isocitric dehydrogenase; mitochondrial; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways