HIPPI (IFT57) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4F6]

CAT#: TA500918BM

Anti-IFT57 (HIPPI) mouse monoclonal antibody, clone OTI4F6 (formerly 4F6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFT57 (RC204116, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFT57. Positive lysates LY402637 (100ug) and LC402637 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IFT57 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IFT57 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500918, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500918, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500918, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IFT57mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500918, Dilution 1:50)

• Anti-IFT57 mouse monoclonal antibody (TA500918) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFT57 (RC204116).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI4F6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: intraflagellar transport 57
• Database Link:
  NP_060480
  Entrez Gene 73916 MouseEntrez Gene 303968 RatEntrez Gene 55081 Human
  UniProt ID Q9NWB7
• Background: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear
• Synonyms: ESRRBL1; HIPPI; MHS4R2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease