CD36 Mouse Monoclonal Antibody [Clone ID: OTI4H7]

CAT#: TA500935S

CD36 mouse monoclonal antibody, clone OTI4H7 (formerly 4H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD36 (RC203254, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36 (1:500).

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  Anti-CD36 mouse monoclonal antibody (TA500935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36 (RC203254).

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  Flow cytometric Analysis of Jurkat cells, using anti-CD36 antibody (TA500935), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-CD36 antibody (TA500935), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-CD36 monoclonal antibody (1:200).

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  Western blot analysis of extracts (35ug) from human placenta tissue by using anti-CD36 monoclonal antibody (1:500).

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500935) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500935) (1:500)

• HEK293T cells transfected with either RC203254 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-CD36 antibody (TA500935), and then analyzed by flow cytometry (1:100).

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Specifications

Product Data

• Clone Name: OTI4H7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:1000, WB: 1:200
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD36 (NP_000063) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.83 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.9 kDa
• Gene Name: CD36 molecule
• Database Link:
  NP_000063
  Entrez Gene 29184 RatEntrez Gene 948 Human
  UniProt ID P16671
• Background: The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: BDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, ECM-receptor interaction, Hematopoietic cell lineage, PPAR signaling pathway