TPMT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A2]

CAT#: TA500966BM

Anti-TPMT mouse monoclonal antibody, clone OTI2A2 (formerly 2A2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT. Positive lysates LY400131 (100ug) and LC400131 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TPMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500966, Dilution 1:50)

• Immunofluorescent staining of HepG2 cells using anti-TPMT mouse monoclonal antibody (TA500966).

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Specifications

Product Data

• Clone Name: OTI2A2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: thiopurine S-methyltransferase
• Database Link:
  NP_000358
  Entrez Gene 7172 Human
  UniProt ID P51580
• Background: This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.
• Synonyms: OTTHUMP00000016076; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine methyltransferase; thiopurine S-methyltransferase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes