MTRF1L Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C8]

CAT#: TA501012BM

Anti-MTRF1L mouse monoclonal antibody, clone OTI1C8 (formerly 1C8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTRF1L (RC204221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTRF1L. Positive lysates LY402734 (100ug) and LC402734 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501012, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501012, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501012, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501012, Dilution 1:50)

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  Anti-MTRF1L mouse monoclonal antibody (TA501012) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MTRF1L (RC204221 ).

• HEK293T cells transfected with either RC204221 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MTRF1L antibody (TA501012), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MTRF1L (NP_061914) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.6 kDa
• Gene Name: mitochondrial translational release factor 1 like
• Database Link:
  NP_061914
  Entrez Gene 54516 Human
  UniProt ID Q9UGC7
• Background: Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG
• Synonyms: HMRF1L; MRF1L; mtRF1a