MTRF1L Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B3]

CAT#: TA501013AM

Anti-MTRF1L mouse monoclonal antibody, clone OTI1B3 (formerly 1B3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTRF1L (RC204221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTRF1L. Positive lysates LY402734 (100ug) and LC402734 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501013, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501013, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501013, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501013, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-MTRF1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501013, Dilution 1:50)

• Anti-MTRF1L mouse monoclonal antibody (TA501013) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MTRF1L (RC204221).

• Flow cytometric analysis of Jurkat cells, using anti-MTRF1L antibody (TA501013), (Red) compared to a nonspecific negative control antibody ([TA50011]) (Blue).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI1B3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MTRF1L (NP_061914) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.6 kDa
• Gene Name: mitochondrial translational release factor 1 like
• Database Link:
  NP_061914
  Entrez Gene 54516 Human
  UniProt ID Q9UGC7
• Background: Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG
• Synonyms: HMRF1L; MRF1L; mtRF1a