TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI2E9]

CAT#: TA501038S

Anti-TUBA8 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8. Positive lysates LY412867 (100ug) and LC412867 (20ug) can be purchased separately from OriGene.

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  Anti-TUBA8 mouse monoclonal antibody (TA501038) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8 (RC211175).

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  Immunoprecipitation (IP) of TUBA8 by using TrueMab monoclonal anti-TUBA8 antibodies (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either pCMV6-ENTRY TUBA8 (RC211175) (Red) or empty vector control plasmid (Blue) were immunostained with anti-TUBA8 mouse monoclonal (TA501038), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E9
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:2000; IF 1:100; IP: 4ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: tubulin alpha 8
• Database Link:
  NP_061816
  Entrez Gene 500377 RatEntrez Gene 51807 Human
  UniProt ID Q9NY65
• Background: Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.
• Synonyms: TUBAL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection