TPMT Mouse Monoclonal Antibody [Clone ID: OTI4C1]

CAT#: TA501054S

Anti-TPMT mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT. Positive lysates LY400131 (100ug) and LC400131 (20ug) can be purchased separately from OriGene.

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  WB analysis of extracts (35ug) from 9 different cell lines by using anti-TPMT monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501054, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501054, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (TA501062, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

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  HEK293T cells transfected with either pCMV6-ENTRY TPMT (RC203309) (Red) or empty vector control plasmid (Blue) were immunostained with anti-TPMT mouse monoclonal (TA501054), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4C1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, Flow 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.88 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: thiopurine S-methyltransferase
• Database Link:
  NP_000358
  Entrez Gene 403536 DogEntrez Gene 7172 Human
  UniProt ID P51580
• Background: This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.
• Synonyms: OTTHUMP00000016076; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine methyltransferase; thiopurine S-methyltransferase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes