PIM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5B5]

CAT#: TA501061AM

PIM2 mouse monoclonal antibody, clone OTI5B5 (formerly 5B5), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2. Positive lysates LY416355 (100ug) and LC416355 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501061)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501061)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501061)

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  Anti-PIM2 mouse monoclonal antibody (TA501061) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2 (RC201933).

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  Immunoprecipitation (IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• HEK293T cells transfected with either RC201933 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody (TA501061), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5B5
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: Pim-2 proto-oncogene, serine/threonine kinase
• Database Link:
  NP_006866
  Entrez Gene 18715 MouseEntrez Gene 317366 RatEntrez Gene 11040 Human
  UniProt ID Q9P1W9
• Background: This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia