DNTTIP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1E2]

CAT#: TA501134AM

Anti-DNTTIP1 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNTTIP1 (RC206867, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNTTIP1. Positive lysates LY403275 (100ug) and LC403275 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNTTIP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-DNTTIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501134, Dilution 1:50)

• Anti-DNTTIP1 mouse monoclonal antibody (TA501134) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNTTIP1 (RC206867).

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Specifications

Product Data

• Clone Name: OTI1E2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNTTIP1 (NP_443183) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.8 kDa
• Gene Name: deoxynucleotidyltransferase terminal interacting protein 1
• Database Link:
  NP_443183
  Entrez Gene 76233 MouseEntrez Gene 171437 RatEntrez Gene 116092 Human
  UniProt ID Q9H147
• Background: DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template
• Synonyms: C20orf167; dJ447F3.4; Tdif1