PKA R2 (PRKAR2A) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1F8]

CAT#: TA501146AM

PRKAR2A (PKA R2 ) mouse monoclonal antibody, clone OTI1F8 (formerly 1F8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A. Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR2A monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PRKAR2A-Knockout HeLa cells (KO, Cat# LC812853) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A monoclonal antibody TA501146 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501146)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501146)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501146)

• Anti-PRKAR2A mouse monoclonal antibody (TA501146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A (RC220376).

• HEK293T cells transfected with either RC220376 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR2A antibody (TA501146), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PRKAR2A antibody (TA501146), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PRKAR2A antibody (TA501146), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI1F8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: protein kinase cAMP-dependent type II regulatory subunit alpha
• Database Link:
  NP_004148
  Entrez Gene 19087 MouseEntrez Gene 29699 RatEntrez Gene 5576 Human
  UniProt ID P13861
• Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq]
• Synonyms: PKR2; PRKAR2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Insulin signaling pathway