cGKI (PRKG1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9A4]

CAT#: TA501208AM

Anti-PRKG1 (cGKI) mouse monoclonal antibody, clone OTI9A4 (formerly 9A4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG1 (RC220986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1. Positive lysates LY416766 (100ug) and LC416766 (20ug) can be purchased separately from OriGene.

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  Anti-PRKG1 mouse monoclonal antibody (TA501208) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKG1 (RC220986).

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  HEK293T cells transfected with either RC220986 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKG1 antibody (TA501208), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-PRKG1 antibody (TA501208), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI9A4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRKG1 (NP_006249) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.6 kDa
• Gene Name: protein kinase cGMP-dependent 1
• Database Link:
  NP_006249
  Entrez Gene 19091 MouseEntrez Gene 54286 RatEntrez Gene 5592 Human
  UniProt ID Q13976
• Synonyms: 1; AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PRKG1B; PRKGR1B

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction, Vascular smooth muscle contraction