ACAT2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7B1]

CAT#: TA501221BM

ACAT2 mouse monoclonal antibody, clone OTI7B1 (formerly 7B1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ACAT2-Knockout HeLa cells (KO, Cat# LC832703) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 monoclonal antibody TA501221 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501221)

• Anti-ACAT2 mouse monoclonal antibody (TA501221) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821).

• Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody (TA501221), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI7B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.2 kDa
• Gene Name: acetyl-CoA acetyltransferase 2
• Database Link:
  NP_005882
  Entrez Gene 308100 RatEntrez Gene 484063 DogEntrez Gene 39 Human
  UniProt ID Q9BWD1
• Background: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq]
• Synonyms: acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; cytosolic acetoacetyl-CoA thiolase; OTTHUMP00000017527

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine, leucine and isoleucine degradation