NXNL2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2B11]

CAT#: TA501246BM

Anti-NXNL2 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NXNL2 (RC205425, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NXNL2. Positive lysates LY408006 (100ug) and LC408006 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NXNL2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501246, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501246, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501246, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501246, Dilution 1:50)

• Immunofluorescent staining of HeLa cells using anti-NXNL2 mouse monoclonal antibody (TA501246).

• Flow cytometric Analysis of Hela cells, using anti-NXNL2 antibody (TA501246), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-NXNL2 antibody (TA501246), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI2B11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NXNL2 (NP_660326) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.5 kDa
• Gene Name: nucleoredoxin like 2
• Database Link:
  NP_660326
  Entrez Gene 75124 MouseEntrez Gene 698533 MonkeyEntrez Gene 158046 Human
  UniProt ID Q5VZ03
• Synonyms: C9orf121; RDCVF2