CD147 (BSG) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4D3]

CAT#: TA501248BM

Anti-BSG (CD147) mouse monoclonal antibody, clone OTI4D3 (formerly 4D3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and BSG-Knockout 293T cells (KO, Cat# LC810433) were separated by SDS-PAGE and immunoblotted with anti-BSG monoclonal antibody TA501248 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501248, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501248, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501248, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501248, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue within the normal limits using anti-CD147 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501248) (1:600).

• Immunohistochemical staining of paraffin-embedded mouse spleen tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501248) (1:600).

• Anti-BSG mouse monoclonal antibody (TA501248) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG (RC219464).

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Specifications

Product Data

• Clone Name: OTI4D3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: basigin (Ok blood group)
• Database Link:
  NP_001719
  Entrez Gene 682 Human
  UniProt ID P35613
• Background: The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 5F7; CD147; EMMPRIN; OK; TCSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane