TACC3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3B1]

CAT#: TA501254AM

TACC3 mouse monoclonal antibody, clone OTI3B1 (formerly 3B1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3. Positive lysates LY416710 (100ug) and LC416710 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501254)

• Anti-TACC3 mouse monoclonal antibody (TA501254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 (RC210754).

• HEK293T cells transfected with either RC210754 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (TA501254), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-TACC3 antibody (TA501254), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI3B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TACC3 (NP_006333) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.2 kDa
• Gene Name: transforming acidic coiled-coil containing protein 3
• Database Link:
  NP_006333
  Entrez Gene 10460 Human
  UniProt ID Q9Y6A5
• Background: The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice. [provided by RefSeq]
• Synonyms: ERIC-1; ERIC1