TACC3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6H4]

CAT#: TA501264BM

TACC3 mouse monoclonal antibody, clone OTI6H4 (formerly 6H4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3. Positive lysates LY416710 (100ug) and LC416710 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TACC3-Knockout 293T cells (KO, Cat# LC812337) were separated by SDS-PAGE and immunoblotted with anti-TACC3 monoclonal antibody TA501264, (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Anti-TACC3 mouse monoclonal antibody (TA501264) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 (RC210754).

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  HEK293T cells transfected with either RC210754 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (TA501264), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6H4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TACC3 (NP_006333) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.2 kDa
• Gene Name: transforming acidic coiled-coil containing protein 3
• Database Link:
  NP_006333
  Entrez Gene 712898 MonkeyEntrez Gene 10460 Human
  UniProt ID Q9Y6A5
• Background: This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells. [provided by RefSeq, Nov
• Synonyms: ERIC-1; ERIC1