HID1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F4]

CAT#: TA501311AM

Anti-C17orf28 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY C17orf28 (Cat# RC206740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28(Cat# TA501311). Positive lysates LY403066 (100ug) and LC403066 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (30ug per lane) from 5 cell lines lysates and Mouse brain tissue lysate by using anti-HID1 monoclonal antibody (TA501311, 1:2000).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-C17orf28 mouse monoclonal antibody (TA501311) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28 (RC206740).

• HEK293T cells transfected with either RC206740 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf28 antibody (TA501311), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-C17orf28 antibody (TA501311), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2F4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C17orf28 (NP_085133) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.6 kDa
• Gene Name: HID1 domain containing
• Database Link:
  NP_085133
  Entrez Gene 287822 RatEntrez Gene 483305 DogEntrez Gene 699954 MonkeyEntrez Gene 283987 Human
  UniProt ID Q8IV36
• Synonyms: C17orf28; DMC1; HID-1