HIBCH Mouse Monoclonal Antibody [Clone ID: OTI3H5]

CAT#: TA501317S

Anti-HIBCH mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)

Product Images

HEK293T cells transfected with either RC209814 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HIBCH antibody (TA501317), and then analyzed by flow cytometry.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH. Positive lysates LY402324 (100ug) and LC402324 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501317, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501317, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HIBCH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501317, Dilution 1:50)

Show all 6 images

Specifications

Product Data

• Clone Name: OTI3H5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, FLOW 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.9 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: 3-hydroxyisobutyryl-CoA hydrolase
• Database Link:
  NP_055177
  Entrez Gene 607040 DogEntrez Gene 26275 Human
  UniProt ID Q6NVY1
• Background: This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
• Synonyms: HIBYLCOAH

Reference Data

• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation